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QuicKey Pro Human D2D(D-Dimer) ELISA Kit

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Product Name QuicKey Pro Human D2D(D-Dimer) ELISA Kit
Price $223.80
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QuicKey Pro Human D2D(D-Dimer) ELISA Kit 수량증가 수량감소 223.8 (  )
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QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay typeSandwich-ELISA
Format96T/48T/24
Assay time1.5h
Detection range125.00-4000 pg/mL
Sensitivity53.10 pg/mL
Sample type &Sample volumeserum and plasma; 50μL
SpecificityThis kit recognizes D2D in samples. No significant cross-reactivity or interference between D2D and analogues was observed.
ReproducibilityBoth intra-CV and inter-CV are < 10%.
ApplicationThis ELISA kit applies to the in vitro quantitative determination of D2D concentrations in serum and plasma.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human D2D. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human D2D are added to the micro ELISA plate wells. Human D2D in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to D2D. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human D2D in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable)96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
24T: 8 wells ×3 strips
96T*5: 5 plates, 96T
2-8℃, 1 months
Reference Standard96T: 2 vials
48T/24T: 1 vial
96T*5: 10 vials
2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)96T: 1 vial, 60 μL
48T/24T: 1 vial, 30 μL
96T*5: 5 vials, 60 μL
2-8℃(Protect from light)
Reference Standard & Sample Diluent96T/48T/24T: 1 vial, 20 mL
96T*5: 5 vials, 20 mL
2-8℃
HRP Linked Ab Diluent96T/48T/24T: 1 vial, 14 mL
96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)96T/48T/24T: 1 vial, 30 mL
96T*5: 5 vials, 30 mL
Substrate Reagent96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃(Protect from light)
Stop Solution96T/48T/24T: 1 vial, 10 mL
96T*5: 5 vials, 10 mL
2-8℃
Plate Sealer96T/48T/24T: 5 pieces
96T*5: 25 pieces
Product Description1 copy
Certificate of Analysis1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level D2D were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level D2D were tested on 3 different plates, 20 replicates in each plate.

 Intra-assay PrecisionInter-assay Precision
Sample123123
n202020202020
Mean (pg/mL)193.00640.271476.29177.05587.911461.37
Standard deviation11.0432.9153.748.8923.6947.64
CV (%)5.725.143.645.024.033.26

Recovery

The recovery of D2D spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample TypeRange (%)Average Recovery (%)
Serum(n=8)93-108100
EDTA plasma(n=8)90-10496

Linearity

Samples were spiked with high concentrations of Human D2D and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsD-Dimer,D dimer,D2D
Research AreaCardiovascular


PAYMENT INFO

We accept the following forms of payment:
- Credit Card
  - Visa
  - MasterCard
  - American Express
  - Discover
- PayPal

DELIVERY INFO

  • 배송 방법 : Parcel Service
  • 배송 지역 : A Region.
  • 배송 비용 : International Shipping Fee
  • 배송 기간 : 3 - 7 days
  • 배송 안내 : Please take into consideration that some products may take additional time for delivery and processing.
    Important: Your order is shipped once your payment has been cleared. Please contact our customer service center if you would like to request an exchange or return on your order.

    - Customs and Duties

    The recipient will be held responsible for international shipments which may be subject to import duties and taxes. Please be advised that these charges are levied by the destination country and we do not have control over them. In the case that the recipient refuses to receive the parcel, the order will be returned to Korea and will not be re-shipped. Relevant expenses (round-trip shipping, customs, etc.) will be deducted from the refund.

EXCHANGE INFO

Exchange and return address
 - [10543] 경기도 고양시 덕양구 청초로 66 (덕은동) 덕은리버워크A-813
 
You are eligible for exchange or return in the following cases:
 - You can exchange or return your order within seven (7) days of receiving your contract details. If the delivery is delayed after you have received your contract details, you can exchange or return your order within seven (7) days upon receipt of your order or after the start of the shipping date.
- You may exchange or return your order within three (3) months following delivery or within thirty (30) days of becoming aware that the goods and services were delivered in specifications that are different from how they were advertised or promised. 
 
You are not eligible for exchange or return in the following cases:
 - If the goods have been damaged or destroyed due to causes attributable to you (However, you are eligible to cancel your order if you had opened the packaging to examine the contents.)
  - If the value of the goods has depreciated significantly due to partial or heavy use
  - If the value of the goods has depreciated significantly because a long period of time has elapsed, making it difficult to resell the goods 
- If the packaging has been damaged for goods that can be reproduced as identical counterfeits 
 - If we have obtained your consent of ineligibility because irrecoverable damage to the seller is expected in the case of an order cancellation for goods that are produced individually for an order.
 - If the provision of digital content has already commenced. (However, this shall not apply to the portion for which the provision of such digital content has not commenced in cases of a contract comprising divisible services or divisible digital content.)
 
 
 
※ You bear the cost of returning your order in the case of an exchange or return whereby the reason for exchange or return is based on your preferences (including exchange for a different color or size, etc.) rather than damage or if the product not matching its description. 
 

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